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  • Description

    (REgularised LIkelihood OptimisatioN) a stand-alone computer program for Maximum A Posteriori (MAP) refinement of (multiple) 3D reconstructions or 2D class averages in cryo-electron microscopy.

  • Usage

    To list all executables provided by RELION, run: $ sbgrid-list relion Copy to clipboard
  • Usage Notes

    SBGrid Usage Info

    RELION versions > 2.0 are GPU-accelerated using Nvidia CUDA on Linux. For general information on running GPU accelerated applications from SBGrid, please see here :

    CUDA builds of RELION are designated by a '_cu' suffix in the version of the application. For example, 3.1.4_cu11.6 is linked against CUDA v11.6 libraries. All builds are single precision on the GPU and can be run on 'consumer-grade' GPU hardware.

    RELION and MPI versions

    RELION uses the 'mpirun' executable to manage MPI "ranks" for parallel processing. RELION versions in SBGrid include OpenMPI in each version. When called from the SBGrid environment, the relion GUI is configured to use the correct version of OpenMPI to manage parallel processing. When calling RELION binaries from the command line, the mpirun.relion executable can be used to ensure the correct version.

    RELION versions > 3 use OpenMPI 3.1.6 and include support for SGE and SLURM schedulers. Older releases use OpenMPI 2.1.2.

    Notes on RELION versions

    RELION releases 4.0.1_cu11.6 4.0.1_cu10.1_legacy 4.0.0_cu11.6 4.0.0_cu10.1_legacy 4.0.0_cu10.1 3.1.4_cu11.6 3.1.4_cu10.1_legacy 3.1.4_cu10.1

    beta releases (likely obsolete) 4.0-beta_cu9.2 4.0-beta2_cu11.5 4.0-beta2_cu11.4.1 4.0-beta2_cu10.2

    Older official releases 3.1.3_cu10.2 3.1.1_cu9.2 3.0.8_cu10.1 2.1_cu8.0 1.4-randomphase3d 1.4b 1.4 1.3

    Legacy version/hardware support
    _legacy releases are compiled for SandyBridge microarchitecture. Other builds are compiled for Haswell.

    (c) Copyright 2023, Structural Biology Grid (

    Last Update to this document 20230308

  • Installation

    Use the following command to install this title with the CLI client: $ sbgrid-cli install relion Copy to clipboard Available operating systems: Linux 64, OS X INTEL
  • Primary Citation*

    S. H. W. Scheres. 2012. A Bayesian View on Cryo-EM Structure Determination. J Mol Biol. 415(2): 406-418.

    S. H. W. Scheres. 2012. RELION: Implementation of a Bayesian approach to cryo-EM structure determination. J Struct Biol. 180(3): 519-530.

    • *Full citation information available through

  • Webinars

    RELION-5.0: Recent developments

    SBGrid webinars are hosted with partial support from the NIH R25 Continuing Education for Structural Biology Mentors #GM151273, in collaboration with Co-PI Jamaine Davis of Meharry Medical College.

    Topic: RELION-5.0: Recent developments

    Presenter: Prof. Sjors Scheres, MRC Laboratory of Molecular Biology

    Host: Shaun Rawson
    Recorded on March 12, 2024

    For more information on RELION:


    Topic: New developments in RELION-4.0
    Presenter: Prof. Sjors Scheres, MRC Laboratory of Molecular Biology

    Host: Jason Key
    Recorded on December 7, 2021


    Topic: RELION - Exploiting prior knowledge about biological macromolecules in cryo-EM structure determination
    Presenter: Dari Kimanius, Ph.D., MRC Laboratory of Molecular Biology

    Host: Shaun Rawson
    Recorded on June 2, 2020

    RELION 3.0

    Topic : RELION-3: new tools for automated high-resolution cryo-EM structure determination
    Presenter: Sjors Scheres, Group Leader, MRC Laboratory of Molecular Biology, UK

    Host: Jason Key / Shaun Rawson
    Recorded: November 6, 2018


    Topic: RELION
    Presenter: Sjors Scheres, Group Leader
    Medical Research Council, The Laboratory of Molecular Biology

    Host: Jason Key
    Recorded on March 24, 2015

  • Keywords

    Electron Microscopy

  • Default Versions

    Linux 64:  4.0.1_cu11.6 (3.1 GB)
    OS X INTEL:  4.0.1 (4.8 GB)

  • Other Versions

      Linux 64:

      1.3 (94.8 MB) , 1.4 (144.8 MB) , 1.4b (112.6 MB) , 1.4-randomphase3d (57.0 MB) , 2.1_cu8.0 (37.2 MB) , 3.0.8_cu10.1 (211.6 MB) , 3.1.1_cu9.2 (2.5 GB) , 3.1.3_cu10.2 (3.4 GB) , 3.1.4_cu10.1 (3.4 GB) , 3.1.4_cu10.1_legacy (10.0 GB) , 3.1.4_cu11.6 (3.9 GB) , 3.1.4_cu11.8 (4.4 GB) , 4.0.0_cu10.1 (2.6 GB) , 4.0.0_cu10.1_legacy (9.2 GB) , 4.0.0_cu11.6 (2.7 GB) , 4.0.1_cu10.1_legacy (9.2 GB) , 4.0.1_cu12.1 (3.1 GB) , 4.0.1_cu12.2 (3.1 GB) , 4.0-beta2_cu10.2 (3.3 GB) , 4.0-beta2_cu11.4.1 (3.4 GB) , 4.0-beta2_cu11.5 (3.4 GB) , 4.0-beta_cu9.2 (4.5 GB) , 5.0b2 (19.4 GB) , 5.0b-3_cu11.8 (21.2 GB) , 5.0-beta2_cu11.8 (19.6 GB) , 5.0-beta3_cu11.8 (21.0 GB) , 5.0-beta3_cu12.2 (21.3 GB) , 5.0-beta_cu10.1 (18.6 GB) , 5.0-beta_cu11.6 (19.5 GB) , 5.0-beta_cu12.2 (27.2 GB)
    • OS X INTEL:

      1.3 (110.1 MB) , 1.4 (113.9 MB) , 2.1 (42.3 MB) , 3.1.4 (317.2 MB) , 3.1.4_m1 (285.3 MB) , 4.0.0 (383.4 MB) , 4.0.0_m1 (343.6 MB) , 4.0.1_m1 (4.4 GB)
    • Common files size: 11.3 GB
  • Developers

    Sjors Scheres