Supported Applications

SPHIRE

Description

(SParx for HIgh Resolution Electron microscopy) for cryoEM using statistical resampling to attain quality assessment and result reproducibility. Includes movie frame alignments (movie), CTF estimation of raw electron micrographs (cter), picking/stack creation (window), reproducible 2-D classification (isac), reproducible initial model generation (viper), automatic gold-standard 3-D refinement (meridien), local resolution estimation and filtering (localres), and up to the 3-D sorting of different conformational states based on the statistical 3-D variability of the data (sort3D).
Usage Notes

Note for running GPU-accelerated ISAC2 (linux-only): sxisac2_gpu.py requires install of cuda 9.0; CUDA_HOME set to '/programs/x86_64-linux/cuda/9.0'; and use of mpirun from eman2 (mpirun.eman2).
Primary Citation*

T. Moriya, M. Saur, M. Stabrin, F. Merino, H. Voicu, Z. Huang, P. A. Penczek, S. Raunser, and C. Gatsogiannis. 2017. High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE. Journal of Visualized Experiments.
- *Full citation information available through
Webinars

Topic: SPHIRE: User-friendly pipeline for near-atomic resolution Cryo-EM structure determination with automatic validation mechanisms.
Speaker: Toshio Moriya, Ph.D. Postdoctoral Fellow, Max Planck Institute of Molecular Physiology

Host: Jason Key
Recorded on: October 10, 2017