Supported Applications


  • Description

    (REgularised LIkelihood OptimisatioN) a stand-alone computer program for Maximum A Posteriori (MAP) refinement of (multiple) 3D reconstructions or 2D class averages in cryo-electron microscopy.

  • Usage Notes

    Recent versions of RELION ( > 2.0 ) are GPU-accelerated using Nvidia CUDA. For general information on running GPU accelerated applications from SBGrid, look here : https://sbgrid.org/wiki/gpu

    CUDA builds of RELION are designated by a '_cu' suffix in the version of the application.
    For example, 2.0.4_cu7.5 is linked against CUDA v7.5 libraries and is not recommended for Pascal architecture Nvidia hardware (GTX 1080 and higher). 2.0.4_cu8.0 is built with CUDA 8.0 which supports Pascal architecture GPUs.
    The '_SP' designation indicates 'single precision' CPU builds. All builds are single precision on the GPU and can be run on 'consumer-grade' GPU hardware.

  • Primary Citation*

    S. H. W. Scheres. 2012. A Bayesian View on Cryo-EM Structure Determination. J Mol Biol. 415(2): 406-418.

    S. H. W. Scheres. 2012. RELION: Implementation of a Bayesian approach to cryo-EM structure determination. J Struct Biol. 180(3): 519-530.

    • *Full citation information available through

  • Citation Note

    If RELION is useful in your work, please use at least one of the primary citations listed below.

  • Webinars

    Topic: RELION
    Presenter: Sjors Scheres, Group Leader
    Medical Research Council, The Laboratory of Molecular Biology

    Host: Jason Key
    Recorded on March 24, 2015

  • Keywords

    Electron Microscopy

  • Default Versions

    Linux 64:  2.0.3_cu8.0
    OS X INTEL:  2.0.6

  • Other Available Versions

      Linux 32:

      1.2, 1.1, 1.0
    • Linux 64:

      2.1-beta_cu8.0, 2.1-beta_cu7.5, 2.1b1_SP, 2.1b1_cu8.0, 2.1b1_cu7.5, 2.0.6_cu8.0, 2.0.6_cu7.5, 2.0.4_cu8.0, 2.0.4_cu7.5, 2.0.3_SP, 2.0.3_cu7.5, 1.4-randomphase3d, 1.4b, 1.4, 1.3
    • OS X INTEL:

      2.1-beta, 2.1b1, 2.0.4, 2.0.3, 1.4, 1.3
  • Developers

    Sjors Scheres.