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GisSPA

  • Description

    a GPU accelerated program, can translationally and rotationally localize the target protein complex in cellular lamellae, as prepared with a focused ion beam, using single cryo-electron microscopy images without tilt-series, and reconstruct the protein complex at near-atomic resolution.

  • Usage

    To list all executables provided by GisSPA, run: $ sbgrid-list gisspa Copy to clipboard

  • Usage Notes

    SBGrid curated GisSPA provides two callable programs: isSPA python.isspa

    Python scripts are located in the scripts directory: /programs/x86_64-linux/gisspa/1.1.2/scripts

  • Installation

    Use the following command to install this title with the CLI client: $ sbgrid-cli install gisspa Copy to clipboard Available operating systems: Linux 64

  • Primary Citation*

    J. Cheng, T. Liu, X. You, F. Zhang, S. F. Sui, X. Wan, and X. Zhang. 2023. Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA. Nature communications. 14(1): 1282.


    • *Full citation information available through

  • Keywords

    Electron Microscopy, Tomography

  • Default Versions

    Linux 64:  1.1.2 (290.9 MB)

  • Developers

    Fa Zhang, Xinzheng Zhang, Sen-Fang Sui, Tong Liu, Xiaohua Wan, Xin You, Jing Cheng

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