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Supported Applications


  • Description

    allows sharpening of high resolution information in 3D maps obtained by electron cryomicroscopy according to the Rosenthal and Henderson approach (JMB 333:721-745, 2003).

  • Usage

    To list all executables provided by EM-BFACTOR, run: $ sbgrid-list em-bfactor Copy to clipboard
  • Usage Notes

    The program accepts 3D maps in any common EM format and accepts FSC curves in the format of most common packages for single particles, cryoEM. As output, the program produces a sharpened map and the Guinier plot that allows assessment of the sharpening.

  • Installation

    Use the following command to install this title with the CLI client: $ sbgrid-cli install em-bfactor Copy to clipboard Available operating systems: Linux 64, OS X INTEL
  • Primary Citation*

    J. Fernandez, D. Luque, J. Caston, and J. Carrascosa. 2008. Sharpening high resolution information in single particle electron cryomicroscopy. J Struct Biol. 164: 170-175.
    (Note: This reference is where EM-BFACTOR was presented to the community.)

    • *Full citation information available through

  • Keywords

    Electron Microscopy

  • Default Versions

    Linux 64:  20140505 (1.9 MB)
    OS X INTEL:  20140505 (1.6 MB)

  • Other Versions

      Linux 64:

      200912 (7.5 MB)
    • OS X INTEL:

      200912 (1.4 MB)
  • Developers

    Jose-Jesus Fernandez