Supported Applications


  • Description

    allows sharpening of high resolution information in 3D maps obtained by electron cryomicroscopy according to the Rosenthal and Henderson approach (JMB 333:721-745, 2003). The program accepts 3D maps in any common EM format and accepts FSC curves in the format of most common packages for single particles, cryoEM. As output, the program produces a sharpened map and the Guinier plot that allows assessment of the sharpening.

  • Primary Citation*

    J. Fernandez, D. Luque, J. Caston, and J. Carrascosa. 2008. Sharpening high resolution information in single particle electron cryomicroscopy. J Struct Biol. 164: 170-175.
    (Note: This reference is where EM-BFACTOR was presented to the community.)

    • *Full citation information available through

  • Categories

    Electron Microscopy

  • Default Versions

    Linux 64 20140505
    OS X INTEL 20140505

  • Other Available Versions

    Linux 32 200912
    Linux 64 200912
    OS X INTEL 200912

  • Developers

    Jose-Jesus Fernandez.