Supported Applications


  • Description

    a novel automated particle picking software based on the deep learning object detection system 'You Only Look Once' (YOLO). CrYOLO is available as standalone program under http://sphire.mpg.de/ and will be part of the image processing workflow in SPHIRE.

  • Installation

    Use the following command to install this title with the CLI client: $ sbgrid-cli install cryolo Copy to clipboard
  • Primary Citation*

    T. Wagner, F. Merino, M. Stabrin, T. Moriya, C. Antoni, A. Apelbaum, P. Hagel, O. Sitsel, T. Raisch, D. Prumbaum, D. Quentin, D. Roderer, S. Tacke, B. Siebolds, E. Schubert, T. R. Shaikh, P. Lill, and C. G. &. S. Raunser. 2019. SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM. Communications Biology. 2(1): 218.

    • *Full citation information available through

  • Webinars

    Topic: crYOLO and its utilization in feedback guided real-time processing
    Speaker: Dr. Thorsten Wagner, Max-Planck-Institute of Molecular Physiology

    Host: Pete Meyer
    Recorded on May 29, 2020

    Topic: Fast, accurate and easy particle picking with crYOLO
    Presenter: Thorsten Wagner, Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology

    Host: Jason Key
    Recorded on: May 28, 2019

  • Keywords

    Electron Microscopy

  • Default Versions

    Linux 64:  1.7.6

  • Other Versions

      Linux 64:

      1.8.0b28-cpu, 1.8.0b28, 1.7.6-cpu, 1.7.5-cpu, 1.7.5, 1.6.1-cpu, 1.6.1
  • Developers

    Stefan Raunser, Thorsten Wagner