Monday, April 23rd at 11:00am EST. Join us for a 30-minute Webinar on "Extending the reach of molecular replacement in Phaser" presented by Randy Read, Wellcome Trust Principal Research Fellow, Department of Haematology, University of Cambridge.

Software information available on the Phaser wiki

Webinar Details: Join the event
Event password: structure

Abstract: We are developing Phaser to implement maximum likelihood methods for solving macromolecular crystal structures by either molecular replacement (the main topic today) or SAD phasing. The use of maximum likelihood is important for two reasons. First, likelihood targets are more sensitive in solving difficult molecular replacement problems than traditional Patterson-based methods. Second, likelihood scores for different possible solutions can be compared directly, providing a convenient basis for automated decision making. Phaser itself is highly automated and, because it can be run directly from within Python scripts, it lends itself to further automation within pipelines. Recent developments are aimed at extending the use of molecular replacement to more difficult problems, e.g. using poorer models or smaller fragments, and simplifying the application of methods combining molecular replacement with experimental phasing.