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MiCSPARC

  • Description

    is a CryoSPARC-based cryo-EM pipeline for microtubule image processing that combines automated filament tracing, particle picking, fast 3D refinement, protofilament-number assignment, alignment, register correction, and seam search to determine structures of decorated and undecorated microtubules.

  • Usage

    To list all executables provided by MiCSPARC, run: $ sbgrid-list micsparc Copy to clipboard

  • Installation

    Use the following command to install this title with the CLI client: $ sbgrid-cli install micsparc Copy to clipboard Available operating systems: Linux 64, OS X INTEL

  • Primary Citation*

    D. Zhang, H. Muñoz-Hernández, P. Filipcik, K. Sejwal, Y. Xu, S. R. Choi, M. O. Steinmetz, and M. Wieczorek. 2026. A cryo-EM processing pipeline for microtubules using CryoSPARC. bioRxiv.


    • *Full citation information available through

  • Keywords

    Electron Microscopy

  • Default Versions

    Linux 64:  1.0 (1.6 GB)
    OS X INTEL:  1.0 (662.5 MB)

  • Developers

    Daniel Zhang, Michal Wieczorek, Michel O. Steinmetz, Hugo Muñoz-Hernández, Pavel Filipcik

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