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MiCSPARC

  • Description

    is a CryoSPARC-based cryo-EM pipeline for microtubule image processing that combines automated filament tracing, particle picking, fast 3D refinement, protofilament-number assignment, alignment, register correction, and seam search to determine structures of decorated and undecorated microtubules. It includes Python utilities for filament extrapolation, reference generation, protofilament assignment, and angular unification, with a conda environment targeting cryosparc-tools 4.7.1. The workflow is designed for end-to-end microtubule reconstruction in CryoSPARC, from curated exposures and template generation through high-resolution protofilament and full microtubule refinement.

  • Usage

    To list all executables provided by MiCSPARC, run: $ sbgrid-list micsparc Copy to clipboard

  • Installation

    Use the following command to install this title with the CLI client: $ sbgrid-cli install micsparc Copy to clipboard Available operating systems: Linux 64, OS X INTEL

  • Keywords

    Electron Microscopy

  • Default Versions

    Linux 64:  1.0 (1.6 GB)
    OS X INTEL:  1.0 (662.5 MB)

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