Search
Webinars Science Tales
Button for mobile navigation
Join us

Supported Applications

magCalEM

  • Description

    Accurate magnification determination for cryoEM. Determining the correct magnified pixel size of single-particle cryoEM micrographs is necessary to maximise resolution and enable accurate model building. We provide a simple python program for determining the absolute magnification in an electron cryomicroscope to a precision of better than 0.5%. The program uses the atomic lattice spacings of crystals of thin and readily available test specimens, such as gold, as an absolute reference to determine magnification for both room temperature and cryogenic imaging. This magnification calibration method provides a definitive reference quantity for data analysis and processing, simplifies the combination of multiple datasets from different microscopes and detectors, and improves the accuracy with which the contrast transfer function of the microscope can be determined.

  • Usage

    To list all executables provided by magCalEM, run: $ sbgrid-list magcalem Copy to clipboard

  • Usage Notes

    To run magCalEM in SBGrid, simply run the command: magcalem If you need to call python to use command line for magCalEM, use python.magcalem

  • Installation

    Use the following command to install this title with the CLI client: $ sbgrid-cli install magcalem Copy to clipboard Available operating systems: Linux 64

  • Primary Citation*

    J. L. Dickerson, E. Leahy, M. J. Peet, K. Naydenova, and C. J. Russo. 2024. Accurate magnification determination for cryoEM using gold. Ultramicroscopy. 256: 113883.


    • *Full citation information available through

  • Keywords

    Electron Microscopy

  • Default Versions

    Linux 64:  1.3.2 (1.1 GB)

  • Developers

    Joshua L. Dickerson, Christopher J Russo

Scroll