SBGrid and NE-CAT are pleased to host a mini-symposium on Low-Resolution Model Building in EM and X-ray Crystallography March 21-22 2019 at Harvard Medical School in Boston. Agenda below.
For information on lodging and location, please see the event website.
March 21-22, 2019, Boston MA
Organizers:
Frank Murphy and Piotr Sliz
Registration Fee: $100 (includes breakfast, lunch, and happy hour reception)
Wednesday, March 20:
7:00pm Optional attendee gathering at the Yard House RSVP here
Thursday, March 21:
Room 250, Tosteson Medical Education Center (TMEC) 260 Longwood Ave
- 8:20- 8:50am: Breakfast
- 8:50- 8:55am: Welcome Remarks (Stephen Blacklow)
- 8:55- 9:00am: Opening Remarks (Frank Murphy & Piotr Sliz)
- 9:00- 9:45am: Opening Keynote: The unique challenges of modeling complexes purified from native sources (Alan Brown)
- 9:45- 10:15am: X-ray Crystallography to Cryo-Electron Microscopy: Software and Data Infrastructure for Structural Biology (Piotr Sliz)
- 10:15-10:30am: Coffee Break
- 10:30-11:00am: Rapid evaluation of EM datasets (Shaun Rawson)
- 11:00-11:45am: Structure determination of the mammalian ryanodine receptor by cryoEM (Oliver Clarke)
- 11:00-11:45am: Homology-based low-resolution refinement and model rebuilding in PDB-REDO (Tassos Perakis)
- 12:30-1:30pm: Lunch
- 1:30-2:00pm: Structure Determination of the DASH/Dam1 Kinetochore Complex by CryoEM (Simon Jeni)
- 2:00-2:30: Analyzing Conformational Dynamics of Flexible Domains with Single-Particle Cryo-EM (Ben Orlando)
- 2:30-2:45: Break
- 2:45-3:30pm: Flexible Fitting into electron densities of heterogenous local resolution - the pros and cones of MD-based refinements (Abhishek Singharoy)
- 3:30-4:00pm: Low-resolution lessons from NE-CAT beamline (Frank Murphy)
- 4:00-4:30pm: High-performance, Low-Resolution compute infrastructure. Clusters, GPU and Clouds (Jason Key)
- 4:30-5:00pm: Structural Determination of Post-translational Translocation Machinery from Yeast (Xudong Wu)
- 5:00-6:00pm: Special Keynote: X-ray crystallography and cryo-EM: synergies and complementarities (Stephen Harrison)
- 6:00-900pm: Reception and Poster Session - TMEC Atrium
- 8:30-9:00am: Breakfast
- 9:00-10:00am: Low-resolution model-building (Tom Terwilliger)
- 10:00-10:45am: X-ray and Cryo-EM refinement (Pavel Afonine)
- 10:45-11:15am: Coffee
- 11:15-12:00pm: Validation for Crystallography and Cryo-EM (Pavel Afonine)
- 12:00-1:00pm: Facilitating Rosetta workflows with Cyrus (Brandon Frenz)
- 1:00-2:00pm: Lunch
- 2:10-2:50pm: (3) 10-min Lightning Talk from Poster Award Recipients
- 3:00-5:00: Open Discussion with Developers, SBGrid, and NE-CAT & EM Facility Tours
- 5:00pm: BCMP Happy Hour/Departure
Friday, March 22:
Room 227, Tosteson Medical Education Center (TMEC) 260 Longwood Ave
See the event website for information on location, lodging, and other logistical details.