SBGrid is pleased to host an Electron Microscopy Data Processing Workshop. This 1-day course will include an introduction to and hands-on tutorial for RELION 2.0 for single particle analysis. The workshop will be held on March 21st at Harvard Medical School in Boston. Preliminary agenda below. For information on lodging and location, please see the event website.
March 21st, 2017
Please contact us at events@sbgrid.org with questions.
Lecturers:
Björn Forsberg, Stockholm University by way of SciLifeLab, Sweden
Dari Kimanius, Stockholm University by way of SciLifeLab, Sweden
Susan Shao, Harvard Medical School
Wei Mi, Harvard Medical School, Maofu Liao's group
Yang Li, Boston Children's Hospital, Hao Wu's group
Arek Kulczyk, Harvard Medical School, Piotr Sliz's group
Jason Key, SBGrid, Harvard Medical School
Organizers:
Susan Shao and Piotr Sliz
Harvard Medical School
Boston, MA
Registration Fee: $100
Fee includes breakfast, lunch, and happy hour reception
See the event website for information on location, lodging, and other logistical details.
Monday, March 20 (optional):
SBGrid 101 - Introduction to Linux command line tools and scripting
6-8pm: Join us for pizza and beer and learn your way around a Linux machine
Tuesday, March 21:
Morning Session (Modell Immunology Center, Fred S. Rosen Lecture Hall, 210 Longwood Ave)
- 8:30- 8:50am: Breakfast
- 8:50- 9:00am: Opening Remarks (Susan Shao)
- 9:00-10:00am: RELION 2.0 introduction (Björn Forsberg and Dari Kimanius)
- 10:00-10:30am: Coffee Break
- 10:30-11:30am: Hardware Considerations (Björn Forsberg and Dari Kimanius)
- 11:30-11:45am: Break - pick up bag lunch
- 11:45-12:45pm Lunchtime Lightning Talks 11:45-12:00: Cryo-EM structure of a translocon in the ERAD
pathway (Wei Mi)
12:05-12:20: Cryo-EM structure of DNAPK (Yang Li)
12:25-12:40: Cryo-EM structure of the replisome (Arek Kulczyk)
Afternoon Tutorial Session
- 1:00- 1:15pm: Introduction and details on cloud computing (Jason Key)
- 1:15- 6:00pm: Tutorial will cover the entire RELION 2.0 workflow, including initial micrograph inspection, CTF estimation, (automated) particle picking, 2D and 3D classification, and 3D high-resolution refinement
- 6:00- 6:15pm Transition to Reception/Poster Session (Modell Immunology Center, Fred S. Rosen Lecture Hall, 210 Longwood Ave)
- 6:15-8:00pm: Reception and Poster Session 6:15-6:50: Group A poster setup (5 min) and presentations (30 min)
- 8:00-10:00pm: Q&A with Björn Forsberg and Dari Kimanius
- 10:00pm: Depart
6:55-7:30: Group B poster setup (5 min) and presentations (30 min)
7:30-8:00: Relax and wrap up.
See the event website for information on location, lodging, and other logistical details.