NE-CAT is pleased to offer our Practical Crystallography Course, June 21-23, 2023 at Harvard Medical School in Boston, Massachusetts. The course will be a three day workshop hosted in conjunction with SBGrid. We are currently planning for three full days with a break for lunch and potentially an afternoon before the start of the workshop to make sure that computers are properly setup. Lunch and coffee breaks will be provided. The workshop remains free, but students are responsible for their own lodging and transportation. All students will need access to SBGrid during the workshop in order to participate. Temporary access to SBGrid can be provided. The workshop is limited to 20 participants. Priority will be given to those with data and/or problematic datasets, but curated datasets are also available for practice.

Apply here.
NE-CAT will notify accepted particiants by mid-May.
See the NE-CAT event website for complete details.

DAY 1: Wednesday, June 21:

•   Data Processing with XDS – Integration
•   Data Processing with POINTLESS, AIMLESS, CAD, TRUNCATE, FREERFLAG - Spacegroups, Scaling, MTZ files
•   Data Analysis, (Molrep, xtriage, matthews) - Non-Crystallographic Symmetry, Pseudo-translation, Twinning

DAY 2: Thursday, June 22:

•   De Novo Phasing using ShelxC/D/E with HKL2MAP
•   AutoSol
•   Making a MR model with AlphaFold2
•   Molecular Replacement using PHASER

DAY 3: Friday, June 23:

•   Is my structure solved? FOM, LLG, RFZ/TFZ, CCall/CCweak
•   Model Building and Refinement (using Coot and phenix.refine)
•   Publishing, data collection statistic table (phenix.table1) including what is acceptable for publication, PYMOL

See the NE-CAT event website for complete details.