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TomoAlign

  • Description

    programs for cryo-tomography tilt-series alignment and subsequent tomographic reconstruction that take into consideration beam-induced sample motion, modeled by means of polynomial surfaces. TomoAlign comprises: 1) tomoalign, for alignment, to determine the basic parameters of the images (rotation, magnification, tilt) along with the polynomial coefficients that model the motion; 2) tomorec to calculate the tomogram from the tilt-series and takes into account the motion estimated during the alignment; 3) auxiliary programs to analyze, transform, or scale the motion parameters.

  • Usage

    To list all executables provided by TomoAlign, run: $ sbgrid-list tomoalign Copy to clipboard

  • Installation

    Use the following command to install this title with the CLI client: $ sbgrid-cli install tomoalign Copy to clipboard Available operating systems: Linux 64, OS X INTEL

  • Primary Citation*

    J. J. Fernández, S. Li, T. A. M. Bharat, and D. A. Agard. 2018. Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion. Journal of Structural Biology. 202(3): 200-209.


    • *Full citation information available through

  • Webinars

    TomoAlign

    Topic: TomoAlign: Consideration of sample motion in cryoET
    Presenter: José-Jesús Fernéndez, Spanish National Centre for Biotechnology, Group Leader

    Host: Jason Key
    Recorded on: April 2, 2019

  • Keywords

    Electron Microscopy

  • Default Versions

    Linux 64:  202103 (9.7 MB)
    OS X INTEL:  202103 (4.5 MB)

  • Other Versions

      Linux 64:

      201901 (8.9 MB)

    • OS X INTEL:

      201901 (3.8 MB)

  • Developers

    Jose-Jesus Fernandez

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